RAD Data comm FLM-1 Guia do Utilizador descarregar pdf (Página 333)

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101

Colony Counting

17-9

Click on the Ignore Region button, then position your cursor on one edge of

the region you want to ignore. Drag your cursor on the image, deﬁning the

full region you want to ignore.

Fig. 17-7. Marking a region to ignore.

As you drag, you will create a “pie slice” marked with red cross-hatching.

Any colonies in this region will not be considered in the ﬁnal count.

When you have deﬁned the region, release the mouse button. If you want to

change the size of the ignored region, position your cursor on the edge of the

pie slice near the rim of the blue circle. Your cursor will change to a

bidirectional arrow, and you can drag the edge of the pie slice.

Colony Count and Adjusted Count

After you have deﬁned a region to ignore, two different counts will appear in

the dialog box: the colony count and the adjusted count.

The colony count is the number of colonies that appear in the deﬁned circle

minus those in the ignored region.

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