RAD Data comm FLM-1 Guia do Utilizador descarregar pdf (Página 295)

100

101

Standards and Band Matching

15-19

Band Set Toolbar

The toolbar in the Band Set form contains all the commands needed for

matching.

Fig. 15-14. Band set toolbar.

Apply to Lane applies the band set to any lane in your gel image. Click on the

button, then click on the lane. The bands in that lane that can be matched will

change to red.

Clear from Lane removes the band set modeling from any lane in your gel

image. Click on the button, then click on the lane to be cleared. The bands in

the lane will change to yellow.

Create Band and Remove Band are standard band commands that have been

included in the toolbar for convenience.

Match is used to deﬁne a band as a new band type. Click on the button, then

click on the unknown (yellow) band to create a new band type. Note that if

you click on a lane that has not been included in a band set, you will be

prompted to create a new band type for every band in the lane.

Unmatch will change a band’s deﬁnition from matched or known to

unknown. Click on this button, then click on the matched (red) or known

(green) band to change it to unknown.

Propagate Band Set applies all the known band types to the bands in a lane,

based on a few manually-assigned bands and the band set model. Click on

this button, then click on the lane. The bands in that lane that can be matched

will change to green to indicate their known status.

Apply to Lane

Clear from Lane

Create Band

Remove Band

Match

Unmatch

Propagate Band Set

Outlier Show band types

Show band models

Standard curve

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