RAD Data comm FLM-1 Guia do Utilizador descarregar pdf (Página 283)

100

101

Standards and Band Matching

15-7

To remove a standard value, click on the triangle button at the beginning of

the row and select Delete from the pop-up box. The remaining standards will

be renumbered.

Applying Standard Values to Lanes

To apply the values to the standard lanes on your image, click on the Apply to

Lane button, then click on a lane.

Fig. 15-7. Click on the Apply to Lane button, then click on the lanes containing standards.

The values will be applied to the bands in the lane you select. Click on any

remaining standard lanes to apply the same values to them.

Note: Generally, the more evenly spaced standard lanes in your gels, the greater

the accuracy of the calculated band values. We recommend a minimum of

two standard lanes per gel. Modeling lines that connect the standard

bands in different lanes are used to compensate for any smiling or

distortion across the gel.

You can also click on the numbered button next to a band value to apply that

value to a particular band in a lane on your image. Click on the button, then

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