RAD Data comm FLM-1 Guia do Utilizador descarregar pdf (Página 296)

Quantity One User Guide

15-20

Outlier excludes a known (green) band from the band set model. However,

the band will still be marked as known.

Show Band Types displays all the red, green, and yellow bands on the image,

with the band type numbers next to the matched bands.

Show Band Models displays the band set modeling lines across the gel image.

Standard Curve displays the Standards Regression Curve (see section 15.1.b).

Click on the button, then click on any lane in the image.

Other Band Set Form Functions

The band set units are displayed in the top half of the form, as is the matching

tolerance used. Tolerance is the minimum spacing between band types that

you speciﬁed when you created the band set.

The Normalization button allows you to pick a speciﬁc band type to

normalize the relative quantities of your other bands against (see section 12.2,

Differential Display, for more information).

Click on the resize button in the lower right corner to reconﬁgure the form to

its smaller, palette version, which displays only the tool buttons and band

type buttons.

To delete the band set, click on the Delete button.

To close the band set, click on OK.

Note that the band set is saved when you save the image.

15.2.c Tips for Gels Without Standards

Ideally, if you are not using standards in your gels, you will have more than

one lane containing a reference sample in your gel. After you’ve used this

reference sample to create a band set, you should apply that band set to other

reference sample lanes using the Propagate Band Set button (in the Band Set

form).

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RAD Data comm FLM-1 Guia do Utilizador Página 296